Simultaneous multiplane imaging with reverberation two-photon microscopy.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32042186.
- Also identified by DOI 10.1038/s41592-019-0728-9 and PMC identifier 8590882.
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Abstract
Multiphoton microscopy has gained enormous popularity because of its unique capacity to provide high-resolution images from deep within scattering tissue. Here, we demonstrate video-rate multiplane imaging with two-photon microscopy by performing near-instantaneous axial scanning while maintaining three-dimensional micrometer-scale resolution. Our technique, termed reverberation microscopy, enables the monitoring of neuronal populations over large depth ranges and can be implemented as a simple add-on to a conventional design.
Medical subject headings
- Brain
- Microscopy, Fluorescence, Multiphoton
- Motor Cortex
- Neurons
- Olfactory Bulb