Detection of Zika Virus Replication in Human Semen by Reverse-Transcription Polymerase Chain Reaction Targeting of Antisense Ribonucleic Acid.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32052024.
- Also identified by DOI 10.1093/infdis/jiaa070.
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Abstract
Persistence of Zika virus (ZIKV) ribonucleic acid (RNA) in semen is common after infection. We designed a reverse-transcription polymerase chain reaction assay that targets antisense ZIKV RNA (asRNA) to assess ZIKV replication competence in ZIKV RNA-positive semen samples. We detected ZIKV asRNA in semen of 9 of 19 men (47.4%) diagnosed with ZIKV infection. All asRNA-positive samples had high ZIKV loads (cycle threshold values <26) and were obtained within 21 days of symptom onset. The sensitivity of the asRNA assay for detection of ZIKV replication was higher than that of conventional virus isolation methods (47.4% vs 21.1%, P = .032).
Medical subject headings
- RNA, Antisense
- RNA, Viral
- Semen
- Virus Replication
- Zika Virus
- Zika Virus Infection