tpHusion: An efficient tool for clonal pH determination in Drosophila.
basic_science · Level V
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- Record sourced from PubMed, PMID 32059043.
- Also identified by DOI 10.1371/journal.pone.0228995 and PMC identifier 7021318.
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Abstract
Genetically encoded pH indicators (GEpHI) have emerged as important tools for investigating intracellular pH (pHi) dynamics in Drosophila. However, most of the indicators are based on the Gal4/UAS binary expression system. Here, we report the generation of a ubiquitously-expressed GEpHI. The fusion protein of super ecliptic pHluorin and FusionRed was cloned under the tubulin promoter (tpHusion) to drive it independently of the Gal4/UAS system. The function of tpHusion was validated in various tissues from different developmental stages of Drosophila. Differences in pHi were also indicated correctly in fixed tissues. Finally, we describe the use of tpHusion for comparative analysis of pHi in manipulated clones and the surrounding cells in epithelial tissues. Our findings establish tpHusion as a robust tool for studying pHi in Drosophila.
Medical subject headings
- Drosophila Proteins
- Embryo, Nonmammalian
- Gene Expression Regulation, Developmental
- Transcription Factors