SPsimSeq: semi-parametric simulation of bulk and single-cell RNA-sequencing data.

Assefa, Alemu Takele; Vandesompele, Jo; Thas, Olivier · Bioinformatics · 2020

basic_science · Level V

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Abstract

SPsimSeq is a semi-parametric simulation method to generate bulk and single-cell RNA-sequencing data. It is designed to simulate gene expression data with maximal retention of the characteristics of real data. It is reasonably flexible to accommodate a wide range of experimental scenarios, including different sample sizes, biological signals (differential expression) and confounding batch effects. The R package and associated documentation is available from https://github.com/CenterForStatistics-UGent/SPsimSeq. Supplementary data are available at Bioinformatics online.

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