Multiplexed mass spectrometry of individual ions improves measurement of proteoforms and their complexes.
basic_science · Level V
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- Record sourced from PubMed, PMID 32123391.
- Also identified by DOI 10.1038/s41592-020-0764-5 and PMC identifier 7131870.
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Abstract
An Orbitrap-based ion analysis procedure determines the direct charge for numerous individual protein ions to generate true mass spectra. This individual ion mass spectrometry (I<sup>2</sup>MS) method for charge detection enables the characterization of highly complicated mixtures of proteoforms and their complexes in both denatured and native modes of operation, revealing information not obtainable by typical measurements of ensembles of ions.
Medical subject headings
- Mass Spectrometry
- Proteins
- Proteomics