Kilohertz two-photon fluorescence microscopy imaging of neural activity in vivo.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32123392.
- Also identified by DOI 10.1038/s41592-020-0762-7 and PMC identifier 7199528.
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Abstract
Understanding information processing in the brain requires monitoring neuronal activity at high spatiotemporal resolution. Using an ultrafast two-photon fluorescence microscope empowered by all-optical laser scanning, we imaged neuronal activity in vivo at up to 3,000 frames per second and submicrometer spatial resolution. This imaging method enabled monitoring of both supra- and subthreshold electrical activity down to 345 μm below the brain surface in head-fixed awake mice.
Medical subject headings
- Brain
- Microscopy, Fluorescence, Multiphoton
- Neurons
- Photons