Rapid mesoscale volumetric imaging of neural activity with synaptic resolution.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32123393.
- Also identified by DOI 10.1038/s41592-020-0760-9 and PMC identifier 7192636.
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Abstract
Imaging neurons and neural circuits over large volumes at high speed and subcellular resolution is a difficult task. Incorporating a Bessel focus module into a two-photon fluorescence mesoscope, we achieved rapid volumetric imaging of neural activity over the mesoscale with synaptic resolution. We applied the technology to calcium imaging of entire dendritic spans of neurons as well as neural ensembles within multiple cortical regions over two hemispheres of the awake mouse brain.
Medical subject headings
- Brain
- Dendrites
- Microscopy, Fluorescence, Multiphoton
- Neurons
- Synapses