Detection of melon necrotic spot virus by one-step reverse transcription loop-mediated isothermal amplification assay.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32134962.
- Also identified by DOI 10.1371/journal.pone.0230023 and PMC identifier 7058275.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Melon necrotic spot virus (MNSV) can cause significant economic losses due to decreased quality in cucurbit crops. The current study is the first to use reverse transcription loop-mediated isothermal amplification (RT-LAMP) for detection of MNSV. A set of four LAMP primers was designed based on the coat protein gene sequence of MNSV, and a RT-LAMP reaction was successfully performed for 1 h at 62°C. The results of RT-LAMP showed high specificity for MNSV and no cross-reaction with other viruses. Compared to traditional reverse transcription-PCR (RT-PCR), the RT-LAMP assay was 103-fold more sensitive in detecting MNSV. Due to its sensitivity, speed and visual assessment, RT-LAMP is appropriate for detecting MNSV in the laboratory.
Medical subject headings
- Nucleic Acid Amplification Techniques
- Reverse Transcription
- Tombusviridae