Multiplexed CRISPR technologies for gene editing and transcriptional regulation.
review · Level V
Where this comes from
- Record sourced from PubMed, PMID 32152313.
- Also identified by DOI 10.1038/s41467-020-15053-x and PMC identifier 7062760.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Multiplexed CRISPR technologies, in which numerous gRNAs or Cas enzymes are expressed at once, have facilitated powerful biological engineering applications, vastly enhancing the scope and efficiencies of genetic editing and transcriptional regulation. In this review, we discuss multiplexed CRISPR technologies and describe methods for the assembly, expression and processing of synthetic guide RNA arrays in vivo. Applications that benefit from multiplexed CRISPR technologies, including cellular recorders, genetic circuits, biosensors, combinatorial genetic perturbations, large-scale genome engineering and the rewiring of metabolic pathways, are highlighted. We also offer a glimpse of emerging challenges and emphasize experimental considerations for future studies.
Medical subject headings
- CRISPR-Cas Systems
- Gene Editing
- Gene Expression Regulation
- Transcription, Genetic