Purification and enrichment of specific chromatin loci.
review · Level V
Where this comes from
- Record sourced from PubMed, PMID 32152500.
- Also identified by DOI 10.1038/s41592-020-0765-4.
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Abstract
Understanding how chromatin is regulated is essential to fully grasp genome biology, and establishing the locus-specific protein composition is a major step toward this goal. Here we explain why the isolation and analysis of a specific chromatin segment are technically challenging, independently of the method. We then describe the published strategies and discuss their advantages and limitations. We conclude by discussing why significant technology developments are required to unambiguously describe the composition of small single loci.
Medical subject headings
- Chromatin
- Chromosome Mapping
- Chromosomes
- Genetic Loci
- Genome