powmic: an R package for power assessment in microbiome case-control studies.

Abstract

Power analysis is essential to decide the sample size of metagenomic sequencing experiments in a case-control study for identifying differentially abundant (DA) microbes. However, the complexity of microbial data characteristics, such as excessive zeros, over-dispersion, compositionality, intrinsically microbial correlations and variable sequencing depths, makes the power analysis particularly challenging because the analytical form is usually unavailable. Here, we develop a simulation-based power assessment strategy and R package powmic, which considers the complexity of microbial data characteristics. A real data example demonstrates the usage of powmic. powmic R package and online tutorial are available at https://github.com/lichen-lab/powmic. chen61@iu.edu. Supplementary data are available at Bioinformatics online.