A 6-nm ultra-photostable DNA FluoroCube for fluorescence imaging.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32203385.
- Also identified by DOI 10.1038/s41592-020-0782-3 and PMC identifier 7138518.
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Abstract
Photobleaching limits extended imaging of fluorescent biological samples. We developed DNA-based 'FluoroCubes' that are similar in size to the green fluorescent protein, have single-point attachment to proteins, have a ~54-fold higher photobleaching lifetime and emit ~43-fold more photons than single organic dyes. We demonstrate that DNA FluoroCubes provide outstanding tools for single-molecule imaging, allowing the tracking of single motor proteins for >800 steps with nanometer precision.
Medical subject headings
- DNA
- Fluorescent Dyes
- Microscopy, Fluorescence
- Optical Imaging