PolishEM: image enhancement in FIB-SEM.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32221611.
- Also identified by DOI 10.1093/bioinformatics/btaa218.
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Abstract
We have developed a software tool to improve the image quality in focused ion beam-scanning electron microscopy (FIB-SEM) stacks: PolishEM. Based on a Gaussian blur model, it automatically estimates and compensates for the blur affecting each individual image. It also includes correction for artifacts commonly arising in FIB-SEM (e.g. curtaining). PolishEM has been optimized for an efficient processing of huge FIB-SEM stacks on standard computers. PolishEM has been developed in C. GPL source code and binaries for Linux, OSX and Windows are available at http://www.cnb.csic.es/%7ejjfernandez/polishem. Supplementary data are available at Bioinformatics online.
Medical subject headings
- Microscopy
- Software