Nitric oxide as a source for bacterial triazole biosynthesis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32235841.
- Also identified by DOI 10.1038/s41467-020-15420-8 and PMC identifier 7109123.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The heterocycle 1,2,3-triazole is among the most versatile chemical scaffolds and has been widely used in diverse fields. However, how nature creates this nitrogen-rich ring system remains unknown. Here, we report the biosynthetic route to the triazole-bearing antimetabolite 8-azaguanine. We reveal that its triazole moiety can be assembled through an enzymatic and non-enzymatic cascade, in which nitric oxide is used as a building block. These results expand our knowledge of the physiological role of nitric oxide synthase in building natural products with a nitrogen-nitrogen bond, and should also inspire the development of synthetic biology approaches for triazole production.
Medical subject headings
- Bacteria
- Nitric Oxide
- Triazoles