Cortical anchoring of the microtubule cytoskeleton is essential for neuron polarity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32293562.
- Also identified by DOI 10.7554/eLife.55111 and PMC identifier 7159925.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The development of a polarized neuron relies on the selective transport of proteins to axons and dendrites. Although it is well known that the microtubule cytoskeleton has a central role in establishing neuronal polarity, how its specific organization is established and maintained is poorly understood. Using the in vivo model system <i>Caenorhabditis elegans</i>, we found that the highly conserved UNC-119 protein provides a link between the membrane-associated Ankyrin (UNC-44) and the microtubule-associated CRMP (UNC-33). Together they form a periodic membrane-associated complex that anchors axonal and dendritic microtubule bundles to the cortex. This anchoring is critical to maintain microtubule organization by opposing kinesin-1 powered microtubule sliding. Disturbing this molecular complex alters neuronal polarity and causes strong developmental defects of the nervous system leading to severely paralyzed animals.
Medical subject headings
- Cell Polarity
- Cytoskeleton
- Microtubules
- Neurons