Artifact-free and high-temporal-resolution in vivo opto-electrophysiology with microLED optoelectrodes.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32345971.
- Also identified by DOI 10.1038/s41467-020-15769-w and PMC identifier 7188816.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The combination of in vivo extracellular recording and genetic-engineering-assisted optical stimulation is a powerful tool for the study of neuronal circuits. Precise analysis of complex neural circuits requires high-density integration of multiple cellular-size light sources and recording electrodes. However, high-density integration inevitably introduces stimulation artifact. We present minimal-stimulation-artifact (miniSTAR) μLED optoelectrodes that enable effective elimination of stimulation artifact. A multi-metal-layer structure with a shielding layer effectively suppresses capacitive coupling of stimulation signals. A heavily boron-doped silicon substrate silences the photovoltaic effect induced from LED illumination. With transient stimulation pulse shaping, we reduced stimulation artifact on miniSTAR μLED optoelectrodes to below 50 μV<sub>pp</sub>, much smaller than a typical spike detection threshold, at optical stimulation of >50 mW mm<sup>-2</sup> irradiance. We demonstrated high-temporal resolution (<1 ms) opto-electrophysiology without any artifact-induced signal quality degradation during in vivo experiments. MiniSTAR μLED optoelectrodes will facilitate functional mapping of local circuits and discoveries in the brain.
Medical subject headings
- Artifacts
- Electrophysiological Phenomena
- Optogenetics