Intracellular calcium leak lowers glucose storage in human muscle, promoting hyperglycemia and diabetes.

Abstract

Most glucose is processed in muscle, for energy or glycogen stores. Malignant Hyperthermia Susceptibility (MHS) exemplifies muscle conditions that increase [Ca²⁺]_cytosol. 42% of MHS patients have hyperglycemia. We show that phosphorylated glycogen phosphorylase (GP<i>a</i>), glycogen synthase (GS<i>a</i>) - respectively activated and inactivated by phosphorylation - and their Ca²⁺-dependent kinase (PhK), are elevated in microsomal extracts from MHS patients' muscle. Glycogen and glucose transporter GLUT4 are decreased. [Ca²⁺]_cytosol, increased to MHS levels, promoted GP phosphorylation. Imaging at ~100 nm resolution located GP<i>a</i> at sarcoplasmic reticulum (SR) junctional cisternae, and <i>apo</i>-GP at Z disk. MHS muscle therefore has a wide-ranging alteration in glucose metabolism: high [Ca²⁺]_cytosol activates PhK, which inhibits GS, activates GP and moves it toward the SR, favoring glycogenolysis. The alterations probably cause these patients' hyperglycemia. For basic studies, MHS emerges as a variable stressor, which forces glucose pathways from the normal to the diseased range, thereby exposing novel metabolic links.