Difference in biophysical properties of cancer-initiating cells in melanoma mutated zebrafish.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32364948.
- Also identified by DOI 10.1016/j.jmbbm.2020.103746 and PMC identifier 7909363.
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Abstract
Despite sharing oncogenetic mutations, only a small number of cells within a given tissue will undergo malignant transformation. Biochemical and physical factors responsible for this cancer-initiation process are not well understood. Here we study biophysical differences of pre-melanoma and melanoma cells in a BRAF<sup>V600E</sup>/P53 zebrafish model. The AFM indentation technique was used to study the cancer-initiating cells while the surrounding melanocytes were the control. We observed a statistically significant decrease in the modulus of elasticity (the effective Young's modulus) of cancer-initiating cells compared to the surrounding melanocytes. No significant differences in the pericellular coat surrounding cells were observed. These results contribute to a better understanding of the factors responsible for the initiation of cancer.
Medical subject headings
- Melanoma
- Zebrafish