Principles of functional neural mapping using an intracortical ultra-density microelectrode array (ultra-density MEA).

Abstract

Intracortical electrical neural recording using solid-state electrodes is a prevalent approach in addressing neurophysiological queries and implementing brain-computer interfacing systems. As a variety of ultra-density microelectrode arrays (ultra-density MEAs) are being created more recently, this paper answers to the rising demand for a more rigorous theory concerning this new type of neural electrode technology, both to guide the proper design and to inform the proper usage. This design and use problem of ultra-density MEAs for functional intracortical neuronal circuit mapping is approached from a signal analysis perspective. Starting with quantitative derivations of key basic concepts, the concept of ultra-density MEA is defined in the context for fully resolving the voltage sources within its view volume. Then, the principle of using such an ultra-density MEA for functional neural mapping is elaborated, and a recursive approach to completely resolve all voltage sources from the ultra-density MEA's recordings is proposed. This approach is further validated using a simulated experiment. Last, the limitations and implications of this work are discussed. MEAs can only be used to map the extracellular somatic action potential (esAP) sources in a neural microcircuit, and AP propagation along individual axons cannot be detected. The key for the ultra-density MEA design is to make sure that each spatial unit of analysis (SUA) contains no more than one active esAP source. The unique neural resolving capability of ultra-density MEAs comparing to conventional MEAs is to be able to spatiotemporally resolve each esAP source within its view volume. The ultimate capability and limitation of neural electrode array technology at such an unprecedented fabrication resolution is unraveled. This work strives to further the discussions on this topic into a more quantitative and rational direction, while providing a theoretical guideline for the rational development and neuroscientific application of an ultra-density MEA for intracortical functional mapping.