Three-photon head-mounted microscope for imaging deep cortical layers in freely moving rats.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32371979.
- Also identified by DOI 10.1038/s41592-020-0817-9.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
We designed a head-mounted three-photon microscope for imaging deep cortical layer neuronal activity in a freely moving rat. Delivery of high-energy excitation pulses at 1,320 nm required both a hollow-core fiber whose transmission properties did not change with fiber movement and dispersion compensation. These developments enabled imaging at >1.1 mm below the cortical surface and stable imaging of layer 5 neuronal activity for >1 h in freely moving rats performing a range of behaviors.
Medical subject headings
- Cerebral Cortex
- Locomotion
- Microscopy, Fluorescence, Multiphoton
- Neuroimaging