An axon-specific expression of HCN channels catalyzes fast action potential signaling in GABAergic interneurons.

Abstract

During high-frequency network activities, fast-spiking, parvalbumin-expressing basket cells (PV⁺-BCs) generate barrages of fast synaptic inhibition to control the probability and precise timing of action potential (AP) initiation in principal neurons. Here we describe a subcellular specialization that contributes to the high speed of synaptic inhibition mediated by PV⁺-BCs. Mapping of hyperpolarization-activated cyclic nucleotide-gated (HCN) channel distribution in rat hippocampal PV⁺-BCs with subcellular patch-clamp methods revealed that functional HCN channels are exclusively expressed in axons and completely absent from somata and dendrites. HCN channels not only enhance AP initiation during sustained high-frequency firing but also speed up the propagation of AP trains in PV⁺-BC axons by dynamically opposing the hyperpolarization produced by Na⁺-K⁺ ATPases. Since axonal AP signaling determines the timing of synaptic communication, the axon-specific expression of HCN channels represents a specialization for PV⁺-BCs to operate at high speed.