Effective coupling of rapid freeze-quench to high-frequency electron paramagnetic resonance.
basic_science · Level V
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- Record sourced from PubMed, PMID 32392255.
- Also identified by DOI 10.1371/journal.pone.0232555 and PMC identifier 7213726.
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Abstract
We report an easy, efficient and reproducible way to prepare Rapid-Freeze-Quench samples in sub-millimeter capillaries and load these into the probe head of a 275 GHz Electron Paramagnetic Resonance spectrometer. Kinetic data obtained for the binding reaction of azide to myoglobin demonstrate the feasibility of the method for high-frequency EPR. Experiments on the same samples at 9.5 GHz show that only a single series of Rapid-Freeze-Quench samples is required for studies at multiple microwave frequencies.
Medical subject headings
- Electron Spin Resonance Spectroscopy