An engineered ScCas9 with broad PAM range and high specificity and activity.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32393822.
- Also identified by DOI 10.1038/s41587-020-0517-0.
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Abstract
CRISPR enzymes require a protospacer-adjacent motif (PAM) near the target cleavage site, constraining the sequences accessible for editing. In the present study, we combine protein motifs from several orthologs to engineer two variants of Streptococcus canis Cas9-Sc<sup>++</sup> and a higher-fidelity mutant HiFi-Sc<sup>++</sup>-that have simultaneously broad 5'-NNG-3' PAM compatibility, robust DNA-cleavage activity and minimal off-target activity. Sc<sup>++</sup> and HiFi-Sc<sup>++</sup> extend the use of CRISPR editing for diverse applications.
Medical subject headings
- Amino Acid Motifs
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Clustered Regularly Interspaced Short Palindromic Repeats