Prime genome editing in rice and wheat.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32393904.
- Also identified by DOI 10.1038/s41587-020-0455-x.
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Abstract
Prime editors, which are CRISPR-Cas9 nickase (H840A)-reverse transcriptase fusions programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells without donor DNA or double-strand breaks. We adapted prime editors for use in plants through codon, promoter, and editing-condition optimization. The resulting suite of plant prime editors enable point mutations, insertions and deletions in rice and wheat protoplasts. Regenerated prime-edited rice plants were obtained at frequencies of up to 21.8%.
Medical subject headings
- Gene Editing
- Oryza
- Triticum