A rationally engineered cytosine base editor retains high on-target activity while reducing both DNA and RNA off-target effects.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32424272.
- Also identified by DOI 10.1038/s41592-020-0832-x.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Cytosine base editors (CBEs) offer a powerful tool for correcting point mutations, yet their DNA and RNA off-target activities have caused concerns in biomedical applications. We describe screens of 23 rationally engineered CBE variants, which reveal mutation residues in the predicted DNA-binding site can dramatically decrease the Cas9-independent off-target effects. Furthermore, we obtained a CBE variant-YE1-BE3-FNLS-that retains high on-target editing efficiency while causing extremely low off-target edits and bystander edits.
Medical subject headings
- CRISPR-Associated Protein 9
- Cytosine
- DNA
- Gene Editing
- RNA