Discrete populations of isotype-switched memory B lymphocytes are maintained in murine spleen and bone marrow.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32444631.
- Also identified by DOI 10.1038/s41467-020-16464-6 and PMC identifier 7244721.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
At present, it is not clear how memory B lymphocytes are maintained over time, and whether only as circulating cells or also residing in particular tissues. Here we describe distinct populations of isotype-switched memory B lymphocytes (Bsm) of murine spleen and bone marrow, identified according to individual transcriptional signature and B cell receptor repertoire. A population of marginal zone-like cells is located exclusively in the spleen, while a population of quiescent Bsm is found only in the bone marrow. Three further resident populations, present in spleen and bone marrow, represent transitional and follicular B cells and B1 cells, respectively. A population representing 10-20% of spleen and bone marrow memory B cells is the only one qualifying as circulating. In the bone marrow, all cells individually dock onto VCAM1<sup>+</sup> stromal cells and, reminiscent of resident memory T and plasma cells, are void of activation, proliferation and mobility.
Medical subject headings
- B-Lymphocytes
- Bone Marrow Cells
- Immunoglobulin Class Switching
- Immunologic Memory
- Spleen