jYCaMP: an optimized calcium indicator for two-photon imaging at fiber laser wavelengths.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32451475.
- Also identified by DOI 10.1038/s41592-020-0835-7 and PMC identifier 7335340.
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Abstract
Femtosecond lasers at fixed wavelengths above 1,000 nm are powerful, stable and inexpensive, making them promising sources for two-photon microscopy. Biosensors optimized for these wavelengths are needed for both next-generation microscopes and affordable turn-key systems. Here we report jYCaMP1, a yellow variant of the calcium indicator jGCaMP7 that outperforms its parent in mice and flies at excitation wavelengths above 1,000 nm and enables improved two-color calcium imaging with red fluorescent protein-based indicators.
Medical subject headings
- Calcium
- Fluorescent Dyes
- Microscopy, Fluorescence, Multiphoton