Illumination guidelines for ultrafast pump-probe experiments by serial femtosecond crystallography.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32451477.
- Also identified by DOI 10.1038/s41592-020-0847-3.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Time-resolved crystallography with X-ray free-electron lasers enables structural characterization of light-induced reactions on ultrafast timescales. To be biologically and chemically relevant, such studies must be carried out in an appropriate photoexcitation regime to avoid multiphoton artifacts, a common issue in recent studies. We describe numerical and experimental approaches to determine how many photons are needed for single-photon excitation in microcrystals, taking into account losses by scattering.
Medical subject headings
- Crystallography, X-Ray
- Photons