Dual base editor catalyzes both cytosine and adenine base conversions in human cells.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32483363.
- Also identified by DOI 10.1038/s41587-020-0527-y.
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Abstract
Although base editors are useful tools for precise genome editing, current base editors can only convert either adenines or cytosines. We developed a dual adenine and cytosine base editor (A&C-BEmax) by fusing both deaminases with a Cas9 nickase to achieve C-to-T and A-to-G conversions at the same target site. Compared to single base editors, A&C-BEmax's activity on adenines is slightly reduced, whereas activity on cytosines is higher and RNA off-target activity is substantially decreased.
Medical subject headings
- Adenine
- CRISPR-Cas Systems
- Cytosine
- Gene Editing