A dual-deaminase CRISPR base editor enables concurrent adenine and cytosine editing.
basic_science · Level V
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- Record sourced from PubMed, PMID 32483364.
- Also identified by DOI 10.1038/s41587-020-0535-y and PMC identifier 7723518.
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Abstract
Existing adenine and cytosine base editors induce only a single type of modification, limiting the range of DNA alterations that can be created. Here we describe a CRISPR-Cas9-based synchronous programmable adenine and cytosine editor (SPACE) that can concurrently introduce A-to-G and C-to-T substitutions with minimal RNA off-target edits. SPACE expands the range of possible DNA sequence alterations, broadening the research applications of CRISPR base editors.
Medical subject headings
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- Cytosine Deaminase
- Gene Editing