Base editors for simultaneous introduction of C-to-T and A-to-G mutations.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32483365.
- Also identified by DOI 10.1038/s41587-020-0509-0.
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Abstract
We describe base editors that combine both cytosine and adenine base-editing functions. A codon-optimized fusion of the cytosine deaminase PmCDA1, the adenosine deaminase TadA and a Cas9 nickase (Target-ACEmax) showed a high median simultaneous C-to-T and A-to-G editing activity at 47 genomic targets. On-target as well as DNA and RNA off-target activities of Target-ACEmax were similar to those of existing single-function base editors.
Medical subject headings
- CRISPR-Associated Protein 9
- CRISPR-Cas Systems
- DNA
- Gene Editing