Comment on "RNA-guided DNA insertion with CRISPR-associated transposases".
editorial · Level V
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- Record sourced from PubMed, PMID 32499410.
- Also identified by DOI 10.1126/science.abb2022.
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Abstract
Strecker <i>et al</i> (Research Articles, 5 July 2019, p. 48) described a system for exploiting a Tn7-type transposon-encoded CRISPR-Cas system to make RNA-guided, programmable insertions. Although this system has great promise, we note that the well-established biochemistry of Tn7 suggests that the particular system used may insert not only the transposon but also the entire donor plasmid.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Transposases