Response to Comment on "RNA-guided DNA insertion with CRISPR-associated transposases".
Level V
Where this comes from
- Record sourced from PubMed, PMID 32499411.
- Also identified by DOI 10.1126/science.abb2920.
- No licence information is recorded for this record.
- Because redistribution is not established, this page shows the abstract only. Follow the links below for the full text.
Abstract
Rice <i>et al</i> suggest that the CRISPR-associated transposase ShCAST system could lead to additional insertion products beyond simple integration of the donor. We clarify the outcomes of ShCAST-mediated insertions in <i>Escherichia coli</i>, which consist of both simple insertions and integration of the donor plasmid. This latter outcome can be avoided by use of a 5' nicked DNA donor.
Medical subject headings
- Clustered Regularly Interspaced Short Palindromic Repeats
- Transposases