Enzyme-Guided Selection and Cascaded Emergence of Nanostructured Constitutional Dynamic Networks.

Abstract

Enzymes (endonucleases) are coupled to constitutional dynamic networks to stimulate the selection of a constituent and cascaded emergence of a new network. This is exemplified with the <i>Eco</i>RI-dictated depletion of a network and selection of a constituent that activates the cascaded emergence of a new network. The new network is further depleted by <i>Hin</i>dIII to a selected constituent that can be coupled to the cascaded emergence of a dynamic network. In addition, upon subjecting a [3 × 3] constitutional dynamic network to endonucleases <i>Eco</i>RI and <i>Hin</i>dIII, the programmed hierarchical selection of [2 × 2] constitutional dynamic networks followed by the biocatalytic selection of a constituent for the subsequent emergence of new networks is demonstrated.