A Magnetic Iron Oxide/Polydopamine Coating Can Improve Osteogenesis of 3D-Printed Porous Titanium Scaffolds with a Static Magnetic Field by Upregulating the TGFβ-Smads Pathway.

Huang, Zhenfei; He, Yu; Chang, Xiao; Liu, Jieying; Yu, Lingjia; Wu, Yuanhao; Li, Yaqian; Tian, Jingjing et al. · Adv Healthc Mater · 2020

basic_science · Level V

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Abstract

3D-printed porous titanium-aluminum-vanadium (Ti6Al4V, pTi) scaffolds offer surgeons a good option for the reconstruction of large bone defects, especially at the load-bearing sites. However, poor osteogenesis limits its application in clinic. In this study, a new magnetic coating is successfully fabricated by codepositing of Fe<sub>3</sub> O<sub>4</sub> nanoparticles and polydopamine (PDA) on the surface of 3D-printed pTi scaffolds, which enhances cell attachment, proliferation, and osteogenic differentiation of hBMSCs in vitro and new bone formation of rabbit femoral bone defects in vivo with/without a static magnetic field (SMF). Furthermore, through proteomic analysis, the enhanced osteogenic effect of the magnetic Fe<sub>3</sub> O<sub>4</sub> /PDA coating with the SMF is found to be related to upregulate the TGFβ-Smads signaling pathway. Therefore, this work provides a simple protocol to improve the osteogenesis of 3D-printed porous pTi scaffolds, which will help their application in clinic.

Medical subject headings