Detection of renal cell carcinoma using plasma and urine cell-free DNA methylomes.

Nuzzo, Pier Vitale; Berchuck, Jacob E; Korthauer, Keegan; Spisak, Sandor; Nassar, Amin H; Abou Alaiwi, Sarah; Chakravarthy, Ankur; Shen, Shu Yi et al. · Nat Med · 2020

cross_sectional · Level IV

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Abstract

Improving early cancer detection has the potential to substantially reduce cancer-related mortality. Cell-free methylated DNA immunoprecipitation and high-throughput sequencing (cfMeDIP-seq) is a highly sensitive assay capable of detecting early-stage tumors. We report accurate classification of patients across all stages of renal cell carcinoma (RCC) in plasma (area under the receiver operating characteristic (AUROC) curve of 0.99) and demonstrate the validity of this assay to identify patients with RCC using urine cell-free DNA (cfDNA; AUROC of 0.86).

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