Multiplexed whole-animal imaging with reversibly switchable optoacoustic proteins.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32582850.
- Also identified by DOI 10.1126/sciadv.aaz6293 and PMC identifier 7292636.
- Licence recorded as CC BY-NC.
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Abstract
We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning-based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.
Medical subject headings
- Photoacoustic Techniques