A Role for Taok2 in Listeria monocytogenes Vacuolar Escape.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32582947.
- Also identified by DOI 10.1093/infdis/jiaa367 and PMC identifier 8922001.
- Licence recorded as CC BY-NC-ND.
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Abstract
The bacterial pathogen Listeria monocytogenes invades host cells, ruptures the internalization vacuole, and reaches the cytosol for replication. A high-content small interfering RNA (siRNA) microscopy screen allowed us to identify epithelial cell factors involved in L. monocytogenes vacuolar rupture, including the serine/threonine kinase Taok2. Kinase activity inhibition using a specific drug validated a role for Taok2 in favoring L. monocytogenes cytoplasmic access. Furthermore, we showed that Taok2 recruitment to L. monocytogenes vacuoles requires the presence of pore-forming toxin listeriolysin O. Overall, our study identified the first set of host factors modulating L. monocytogenes vacuolar rupture and cytoplasmic access in epithelial cells.
Medical subject headings
- Listeria monocytogenes
- Listeriosis