Up to 100-fold speed-up and multiplexing in optimized DNA-PAINT.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32601424.
- Also identified by DOI 10.1038/s41592-020-0869-x and PMC identifier 7610413.
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Abstract
DNA-PAINT's imaging speed has recently been significantly enhanced by optimized sequence design and buffer conditions. However, this implementation has not reached an ultimate speed limit and is only applicable to imaging of single targets. To further improve acquisition speed, we introduce concatenated, periodic DNA sequence motifs, yielding up to 100-fold-faster sampling in comparison to traditional DNA-PAINT. We extend this approach to six orthogonal sequence motifs, now enabling speed-optimized multiplexed imaging.
Medical subject headings
- DNA
- Molecular Imaging