TNFAIP8 family gene expressions in the mouse tail intervertebral disc injury model.

Abstract

The TNF-α-induced protein-8 (TNFAIP8, also known as TIPE) family of molecules comprises four members: TNFAIP8 and TIPEs1-3. Since the first description of these proteins, their roles in fine-tuning inflammation and in directing leukocyte migration have been described in several organ systems. However, their relationship with intervertebral disc (IVD) is unknown. Here, we describe the expression of TNFAIP8 family genes in the nucleus pulposus (NP) and annulus fibrosus (AF) of the normal adult murine IVD. We further describe the expression of these genes in the injured male and female murine IVD. <i>Tnfaip8</i> gene expression was decreased, and <i>Tipe1</i> gene expression was essentially unchanged, in response to injury. <i>Tipe2</i> and <i>Tipe3</i> gene expression was markedly elevated in response to IVD injury, along with those encoding known inflammatory markers (ie, <i>Tnfa, Il6, Cxcl1</i>, and <i>Adam8</i>). Additionally, sex-related differences were also observed for some of these genes in intact and injured mouse IVDs. Future studies include examining tissue distribution of TNFAIP8 family proteins and identifying cells that produce them. In addition, examining mice that are deficient in TNFAIP8 molecules, in relation to gene expression, tissue morphology and mouse behavior, may further delineate the roles of these molecules in IVD inflammation and degeneration.