One for All: A Pooled Approach to Classify Functional Impacts of Multiple Mutations.

Jourdon, Alexandre; Vaccarino, Flora M · Cell Stem Cell · 2020

basic_science · Level V

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Abstract

Understanding common biological consequences of heterogenous mutations in complex polygenic conditions is challenging. In this issue of Cell Stem Cell, Cederquist et al. (2020) implement an in vitro pooled assay where 30 high-confidence ASD mutations engineered in subclones of a human pluripotent stem cell line can be investigated in parallel to reveal their effects on prefrontal cortex neurogenesis.

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