Cryo-EM structure of arabinosyltransferase EmbB from Mycobacterium smegmatis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32636380.
- Also identified by DOI 10.1038/s41467-020-17202-8 and PMC identifier 7341804.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Arabinosyltransferase B (EmbB) belongs to a family of membrane-bound glycosyltransferases that build the lipidated polysaccharides of the mycobacterial cell envelope, and are targets of anti-tuberculosis drug ethambutol. We present the 3.3 Å resolution single-particle cryo-electron microscopy structure of Mycobacterium smegmatis EmbB, providing insights on substrate binding and reaction mechanism. Mutations that confer ethambutol resistance map mostly around the putative active site, suggesting this to be the location of drug binding.
Medical subject headings
- Mycobacterium smegmatis
- Pentosyltransferases