Structure of the yeast Swi/Snf complex in a nucleosome free state.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32636384.
- Also identified by DOI 10.1038/s41467-020-17229-x and PMC identifier 7340788.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
SWI/SNF remodelers play a key role in regulating chromatin architecture and gene expression. Here, we report the cryo-EM structure of the Saccharomyces cerevisiae Swi/Snf complex in a nucleosome-free state. The structure consists of a stable triangular base module and a flexible Arp module. The conserved subunits Swi1 and Swi3 form the backbone of the complex and closely interact with other components. Snf6, which is specific for yeast Swi/Snf complex, stabilizes the binding of the ATPase-containing subunit Snf2 to the base module. Comparison of the yeast Swi/Snf and RSC complexes reveals conserved structural features that govern the assembly and function of these two subfamilies of chromatin remodelers. Our findings complement those from recent structures of the yeast and human chromatin remodelers and provide further insights into the assembly and function of the SWI/SNF remodelers.
Medical subject headings
- Adenosine Triphosphatases
- Chromatin
- Chromosomal Proteins, Non-Histone
- Nuclear Proteins
- Repressor Proteins
- Saccharomyces cerevisiae Proteins
- Transcription Factors