Accessing unexplored regions of sequence space in directed enzyme evolution via insertion/deletion mutagenesis.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32651386.
- Also identified by DOI 10.1038/s41467-020-17061-3 and PMC identifier 7351745.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Insertions and deletions (InDels) are frequently observed in natural protein evolution, yet their potential remains untapped in laboratory evolution. Here we introduce a transposon-based mutagenesis approach (TRIAD) to generate libraries of random variants with short in-frame InDels, and screen TRIAD libraries to evolve a promiscuous arylesterase activity in a phosphotriesterase. The evolution exhibits features that differ from previous point mutagenesis campaigns: while the average activity of TRIAD variants is more compromised, a larger proportion has successfully adapted for the activity. Different functional profiles emerge: (i) both strong and weak trade-off between activities are observed; (ii) trade-off is more severe (20- to 35-fold increased k<sub>cat</sub>/K<sub>M</sub> in arylesterase with 60-400-fold decreases in phosphotriesterase activity) and (iii) improvements are present in k<sub>cat</sub> rather than just in K<sub>M</sub>, suggesting adaptive solutions. These distinct features make TRIAD an alternative to widely used point mutagenesis, accessing functional innovations and traversing unexplored fitness landscape regions.
Medical subject headings
- INDEL Mutation