Flow cytometric identification of T<sub>fh</sub>13 cells in mouse and human.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32709424.
- Also identified by DOI 10.1016/j.jaci.2020.04.063 and PMC identifier 7854882.
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Abstract
Anaphylaxis is a life-threatening allergic reaction caused by cross-linking of high-affinity IgE antibodies on the surface of mast cells and basophils. Understanding the cellular mechanisms that lead to high-affinity IgE production is required to develop better therapeutics for preventing this severe reaction. A recently discovered population of T follicular helper T<sub>fh</sub>13 cells regulates the production of high-affinity IgE in mouse models of allergy and can also be found in patients with allergies with IgE antibodies against food or aeroallergens. Here we describe optimized protocols for identifying T<sub>fh</sub>13 cells in both mice and humans.
Medical subject headings
- Cell Separation
- Flow Cytometry
- T Follicular Helper Cells
- T-Lymphocyte Subsets