Isolating live cell clones from barcoded populations using CRISPRa-inducible reporters.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32719478.
- Also identified by DOI 10.1038/s41587-020-0614-0 and PMC identifier 7616981.
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Abstract
We developed a functional lineage tracing tool termed CaTCH (CRISPRa tracing of clones in heterogeneous cell populations). CaTCH combines precise clonal tracing of millions of cells with the ability to retrospectively isolate founding clones alive before and during selection, allowing functional experiments. Using CaTCH, we captured rare clones representing as little as 0.001% of a population and investigated the emergence of resistance to targeted melanoma therapy in vivo.
Medical subject headings
- CRISPR-Cas Systems
- Cell Separation
- Clone Cells
- Genes, Reporter