Light microscopy of proteins in their ultrastructural context.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32737322.
- Also identified by DOI 10.1038/s41467-020-17523-8 and PMC identifier 7395138.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
Resolving the distribution of specific proteins at the nanoscale in the ultrastructural context of the cell is a major challenge in fluorescence microscopy. We report the discovery of a new principle for an optical contrast equivalent to electron microscopy (EM) which reveals the ultrastructural context of the cells with a conventional confocal microscope. By decrowding the intracellular space through 13 to 21-fold physical expansion while simultaneously retaining the proteins, bulk (pan) labeling of the proteome resolves local protein densities and reveals the cellular nanoarchitecture by standard light microscopy.
Medical subject headings
- Imaging, Three-Dimensional
- Microscopy, Fluorescence
- Proteome
- Staining and Labeling