Chemical proteomics tracks virus entry and uncovers NCAM1 as Zika virus receptor.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32753727.
- Also identified by DOI 10.1038/s41467-020-17638-y and PMC identifier 7403387.
- Licence recorded as CC BY.
- The licence permits redistribution, so the abstract is shown in full and the full text is available from the publisher.
Abstract
The outbreak of Zika virus (ZIKV) in 2016 created worldwide health emergency which demand urgent research efforts on understanding the virus biology and developing therapeutic strategies. Here, we present a time-resolved chemical proteomic strategy to track the early-stage entry of ZIKV into host cells. ZIKV was labeled on its surface with a chemical probe, which carries a photocrosslinker to covalently link virus-interacting proteins in living cells on UV exposure at different time points, and a biotin tag for subsequent enrichment and mass spectrometric identification of the receptor or other host proteins critical for virus internalization. We identified Neural Cell Adhesion Molecule (NCAM1) as a potential ZIKV receptor and further validated it through overexpression, knockout, and inhibition of NCAM1 in Vero cells and human glioblastoma cells U-251 MG. Collectively, the strategy can serve as a universal tool to map virus entry pathways and uncover key interacting proteins.
Medical subject headings
- Neural Cell Adhesion Molecules
- Proteomics
- Receptors, Virus
- Virus Internalization
- Virus Replication
- Zika Virus