Actin chromobody imaging reveals sub-organellar actin dynamics.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32778832.
- Also identified by DOI 10.1038/s41592-020-0926-5 and PMC identifier 7746311.
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Abstract
The actin cytoskeleton plays multiple critical roles in cells, from cell migration to organelle dynamics. The small and transient actin structures regulating organelle dynamics are challenging to detect with fluorescence microscopy, making it difficult to determine whether actin filaments are directly associated with specific membranes. To address these limitations, we developed fluorescent-protein-tagged actin nanobodies, termed 'actin chromobodies' (ACs), targeted to organelle membranes to enable high-resolution imaging of sub-organellar actin dynamics.
Medical subject headings
- Actin Cytoskeleton
- Optical Imaging