Time-resolved cryo-EM using Spotiton.
basic_science · Level V
Where this comes from
- Record sourced from PubMed, PMID 32778833.
- Also identified by DOI 10.1038/s41592-020-0925-6 and PMC identifier 7799389.
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Abstract
We present an approach for preparing cryo-electron microscopy (cryo-EM) grids to study short-lived molecular states. Using piezoelectric dispensing, two independent streams of ~50-pl droplets of sample are deposited within 10 ms of each other onto the surface of a nanowire EM grid, and the mixing reaction stops when the grid is vitrified in liquid ethane ~100 ms later. We demonstrate this approach for four biological systems where short-lived states are of high interest.
Medical subject headings
- Cryoelectron Microscopy